We study redox biology in zebrafish and cellular models using redox sensitive probes and antioxidant transcription factor reporter constructs that are compartmentally localized in the cell. Through these and other techniques, we can determine the redox states in phases of development for different organs and various cell types and how the signaling pathways are regulated during development and differentiation.
We study the role that superoxide dismutase play in valproic acid induced reactive oxygen species. We take large scope approach to measure superoxide dismutase activity within a cell. We use epinephrine assays to measure whole cell superoxide dismutase activity and O2 respirometry to see the effects on the electron transport chain.
Birth defects are the leading contributor to infant mortality during the first year of life. Unfortunately, the mechanistic origins of most birth defects remain largely unknown. Recent research suggests, however, that certain teratogens elicit their harmful effects primarily through oxidative stress. I study how manipulation of specific antioxidant pathways combats teratogen-induced redox disruptions, decreasing the prevalence of birth defects in the developing embryo.
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